10th Structural Biology Club of the Czech Society for Structural Biology
29 June 2022, 1 pm
with the following scientific talks kindly delivered by our guests:
Crystal structures and dynamics: Normal mode analysis
Presented by Jacob A. Bauer, Institute of Molecular Biology, Slovak Academy of Sciences
In most protein structure analyses, a large amount of effort is devoted to describing the structural details of the protein, but much less effort is given to characterize the possible range of dynamic motion available to it. Normal mode analysis is a computationally undemanding method that describes the dynamic states available to a given protein about a particular minimum conformation, and many routine structural studies would probably benefit from including it into their analysis. A few examples drawn from the recent literature will be given in illustration. The range of available motions depends mostly on the shape of the given molecule; this will be briefly explored.
From local tau structures to considerations about its conformational ensemble
Presented by Ondrej Cehlár, Institute of Neuroimmunology, Slovak Academy of Sciences
Structural features of the intrinsically disordered protein tau can be best described as a conformational ensemble of interconverting transient structures. In the course of a neurodegenerative tauopathy, specific structural changes leading to subsequent filament formation occur. Particular local conformations of tau protein can be stabilized in the complex with respective anti-tau antibody Fab fragments and the structure can be solved by the means of X-ray crystallography. In our work we combine the structural knowledge about tau local conformations with biophysical and in-cell measurements of tau interactions with its binding partners that can yield information about features of its conformational ensemble, and which will be discussed here.
Moderator: Rostislav Škrabana, Institute of Neuroimmunology SAS, Bratislava
Please, join us on this Zoom link (join 5-10 minutes before the beginning)
Meeting ID: 934 8283 6889