14th Structural Biology Club of the Czech Society for Structural Biology
22 February 2023, 13:00
with the following scientific talks kindly delivered by our guests:
Bias, variance and map validation in Single Particle Analysis by CryoEM
Presented by Jose Luis Vilas, Spanish National Centre for Biotechnology, CSIC, Madrid, Spain
Single Particle Analysis by CryoEM has established as a mature technique to elucidate the threedimensional structure of biological macromolecules. Once the experimental data is acquired, reconstructing the macromolecule’s map involves the estimation of millions of parameters. Due to the low Signal‐to‐Noise Ratio, this estimation is prone to mistakes. Depending on the error size, these mistakes may result into small perturbations in the reconstructed map (variance) or large artifacts
(bias). In this talk we will discuss different kinds of mistakes that can be committed and how to identify and tackle them.
Backtracking of influenza polymerase upon consecutive incorporation of nucleoside analogue T1106 directly observed by cryo‐EM
Presented by Tomáš Kouba, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague
The broad‐spectrum antiviral, nucleoside analog T1106, is incorporated into nascent RNA by viral polymerases. We report cryo‐EM structures of influenza polymerase, showing that consecutive, but not single, T1106 incorporation causes polymerase stalling, backtracking, and chain termination. These results detail one of the diverse mechanisms by which nucleoside analog drugs inhibit viral RNA synthesis.
Moderator: Jiří Nováček, CEITEC, Brno
Please, join us on this Zoom link (join 5‐10 minutes before the beginning)
Meeting ID: 988 6741 9047