Institute of Biotechnology AS CR,
Institute of Microbiology AS CR,
Institute of Nanobiology and Structural Biology, GCRC AS CR,
South Bohemian University in České Budějovice,
Czech Society for Structural Biology
proudly organize
XII Discussions in Structural Molecular Biology
Nové Hrady, Thursday March 13 – Saturday March 15 2014
Please, note early start of the meeting at 13:00
XII Discussions will celebrate the International Year of Crystallography
Program
Thursday, March 13
10:30 — 12:00 Registration at Nový Zámek
12:00 — 12:45 Accommodation in hotels
13:00 – 14:15 Special Session celebrating IYCr A: Chairperson Jan Dohnálek
Plenary Lecture to Celebrate the International Year of Crystallography
David Stuart: Challenges of virus crystallography
14:15 – 14:45 Coffee break
14:45 – 16:05 Special Session celebrating IYCr B: Chairperson Bohdan Schneider
Radomír Kužel: 2014 – International Year of Crystallography
Jindřich Hašek: International appreciation of crystallography
Imrich Barak: User consortium of serial femtosecond crystallography – Slovak involvement
Jan Dohnálek: Methods of structural biology of Instruct available to Czech researchers
16:05 – 16:30 Coffee break
16:30 – 18:00 Session I: Chairperson Petr Novák
Daniel Němeček: Application of Difference 3D Electron Microscopy to Identify DSS1 Subunits and Induced Conformational Changes in the Complex with Rad52
Tanvir Shaikh: Initial bridges between the two ribosomal subunits are formed within 9.4 milliseconds: A time-resolved cryo-EM study
Zdeněk Kukačka: Chemical Cross-linking and Hydrogen-Deuterium Exchange –Tools for Precise Definition of Human Haptoglobin Structure
Evžen Bouřa: Combination of Xtal, SAXS, DEER and FRET data – the structure of ESCRT-I/II supercomplex and implications for membrane budding (30′)
18:00 – 21:00 Dinner
Friday , March 14
08:00 Breakfast
09:00 – 10:00 Session II: Chairperson Tomáš Obšil
Vernon Smith: Improvements to Data Quality with the PHOTON 100 detector in Shutterless Mode
Jiří Maršík: Latest Developments for Life Science from Rigaku
Jiří Brynda: X-ray Structural Analysis of Carbonic Anhydrase and Carborane-Based Inhibitors Complexes
10:00 – 10:20 Coffee break
10:20 – 12:00 Session III: Chairperson Evžen Bouřa
Kvido Strisovsky: Crystal structures of rhomboid intramembrane protease bound to substrate peptides elucidate the basis of substrate specificity and mechanism (30′)
David Kopečný: Family of plant nucleoside N-ribohydrolases
Veronika Lišková: Engineering enzyme access tunnels for protein stabilization
Sérgio Marques: Investigation of gating mechanisms in the access tunnels of haloalkane dehalogenases (30′)
12:10 – 13:30 Lunch
13:45 – 15:35 Session IV: Chairperson Daniel Němeček
Moran Jerabek: MicroScale Thermophoresis: Interaction analysis and beyond (30′)
Lucie Bednarova: Raman microspectroscopy of Candida albicans vacuoles
Martin Golan: Theoretical and experimental study of charge transfer through DNA: impact of mercury attached to mismatched base pairs
Tatsiana Charnavets: Structure, stability and thermodynamic characteristics of single stranded DNA in repetitive extragenic palindromic elements
Josef Houser: RSL lectin – a complex approach to study carbohydrate-protein interaction
15:35 – 16:00 Coffee break
16:00 – 17:40 Session V: Chairperson Richard Hrabal
Matej Zábrady: Recombinant antibodies for in vivo applications
Jakub Ptáček: Selection and characterization of Anticalins specific for human glutamate carboxypeptidase II
Miroslava Kopecka: Role of EF-hand motif in the activation of neutral trehalase
Dalibor Kosek: Characterization of interactions between protein kinase ASK1 and its binding partners.
Ivana Nemcovicova: Cytomegalovirus gpUL141 exhibits unique receptor-binding domain to inhibit cell surface expression of CD155 and TRAIL death receptors
17:45 – 19:00 Dinner
19:00 – 20:00 General Assembly of the Czech Society for Structural Biology. Meeting will be held in the Czech and/or Slovak languages
Program Valného shromáždění ČSSB
1. Výroční zpráva Rady ČSSB o činnosti a hospodaření
2. Schválení výroční zprávy Rady ČSSB o činnosti a hospodaření
3. Zpráva revizní komise
4. Situace s budováním evropských infrastrukturních center
5. Členství naší společnosti v Radě vědeckých společností (RVS)
6. Nový Občanský zákoník – změny vyplývající pro ČSSB
7. Různé
8. Diskuse
20:15 – 22:45 Poster session – posters are listed below
Saturday, March 15
08:00 Breakfast
09:00 – 10:00 Session VI: Chairperson Rudiger Ettrich
Katsiaryna Tratsiak: Haloalkane dehalogenases: DmxA from Marinobacter sp. ELB 17 and DpcA from Psychrobacter cryohalolentis K5, from the crystallization to the structure analysis
Radka Chaloupková: Comparison of catalytic performance of haloalkane dehalogenases in deep eutectic and organic co-solvents
Petra Szelcsányiová: Modification of substrate specificity and catalytic activity of haloalkane dehalogenase linb by opening de novo access tunnels
10:00 – 10:20 Coffee break
10:20 – 12:00 Session VII: Chairperson Jiří Černý
Rudiger Ettrich: Charybdotoxin unbinding from the mKv1.3 potassium channel: A Combined computational and experimental study (30′)
Saurabh Kumar: Pandey Binding competent states for L-arginine in E. coli arginine repressor apoprotein
Hedi Hegyi: Domain Integrity Verification of Alternative Splicing – the DIVAS server
Bohdan Schneider: Dynamics of biomolecules estimated by crystallographic B-factors (30′)
12:00 – 12:05 Closing remarks
12:15 Lunch
- All talks labeled (30′) take 30 minutes, all others take 20 minutes. These times will be observed and include discussion, leave at least 5 minutes to your colleagegues!
Poster session starts on Friday March 14 at 20:15
Posters (alphabetically)
| ID | First | Last | Title |
| 12 | Adriana | Baumlova | Structural characterization of phosphatidylinositol 4-phosphate IIalpha |
| 37 | Lada | Biedermannová | Hydrated amino acid rotamers |
| 34 | Kristýna | Boušová | Calmodulin and S100A1 protein interact with the intracellular termini of the TRPM4 channel |
| 94 | Jiří | Bufka | Synthesis and Spectroscopy of Derivatives of the Fluorescent Protein Chromophore |
| 52 | Zuzana | Chromikova | Crucial role of SpoIIE in Bacillus subtilis cell differentiation |
| 96 | Zdeněk | Chval | Molecular dynamics calculations of the Cy3 and Cy5 cyanine dyes terminally attached to DNA |
| 70 | Ladislav | Cséfalvay | Purification and Initial Imaging of the type I DNA Restriction-Modification Enzyme EcoR124I by Electron Microscopy |
| 110 | Oksana | Degtjarik | Crystallization and structural characterization of LinB86 haloalkane dehalogenase mutant |
| 114 | Radka | Dopitová | Crystal Structure of Phosphotransmitter AHP2 and Modeling of its Interaction with Sensor Histidine Kinase CKI1 – Towards Elucidating Molecular Recognition in the Multistep Phosphorelay Signaling in Plants |
| 95 | David | Dufour Rausell | RNA structure prediction by knowledge-based statistical potentials and fragment assembly |
| 97 | Olga | Dvořáčková | Influence of substitution effects in the pyridine ring on the reactivity of the trans-[Pt(NH3)2(pyr)Cl]+ complex. |
| 39 | Karla | Fejfarová | Crystallization and preliminary X-ray diffraction analysis of nepenthesin-1 |
| 78 | Jan | Frömmel | Aminoaldehyde dehydrogenase isoenzymes from Pisum sativum oxidize N-acylated aminoaldehydes |
| 100 | Pavel | Grinkevich | Structure and function of the C-terminal helical domain of the motor subunit HsdR from the type I restriction-modification system EcoR124I |
| 108 | Dipanjan | Guha | Initial imaging and structural analysis of the hamartin-tuberin complex |
| 111 | Jiří | Heller | Photosystem II PsbO protein from higher plants |
| 62 | Petr | Hošek | Moving hills – new parallelizable metadynamic method with fixed amount of bias potential |
| 72 | Dominik | Hrebík | Interactions between receiver domain of cytokinin receptor CKI1RD and AHP protein from Arabidopsis thaliana |
| 32 | Iuliia | Iermak | Crystallization of glyceraldehyde dehydrogenase from Thermoplasma acidophilum |
| 40 | Michaela | Jirku | Characterization of the calmodulin and S100A1 binding domains on the N-terminus of TRPM1 receptor |
| 42 | Miroslava | Kacirova | Structural studies on Pdc/14-3-3 protein complex |
| 80 | Radka | Končitíková | Structure-function study on enzymes linked to cytokinin metabolism – aldehyde dehydrogenases and nucleoside N-ribohydrolases |
| 102 | Terézia | Kovaľová | Structural and catalytic properties of α-L-fucosidase from Paenibacillus thiaminolyticus |
| 107 | Daniela | Krajčíková | Studies of Self-assembly Properties of Bacillus Spore Coat Proteins |
| 55 | Tomáš | Kroupa | Interaction of M-PMV matrix protein mutants with membranes |
| 68 | Monika | Krupová | Detection of Molecular Chirality in Lanthanide Complexes by the Raman Optical Activity |
| 24 | Ivana | Kuta Smatanova | Protein crystallization projects at the Faculty of Science of the USB CB |
| 41 | Salome | Kylarova | Structural characterization of the thioredoxin-binding domain of protein kinase ASK1 and its interaction with thioredoxin |
| 49 | Lucie | Marečková | KLK2- and KLK11-binding proteins – The novel class of recombinant capture ligands for improved Prostate Cancer diagnostics |
| 112 | Zora | Novakova | The role of the non-pharmacophore pocket of glutamate carboxypeptidase II in the design of small-molecule inhibitors |
| 60 | Jiri | Pavlicek | Crystal structures of complexes of GCPII with P1’-diversified urea-based inhibitors |
| 109 | Miroslav | Peterek | Assembly and molecular architecture of PI4K IIIa complexes |
| 113 | Maria | Plevaka | Crystallization and preliminary diffraction analysis of DhaA57, DhaA80 and DhaA106 mutants from Rhodococcus rhodochrous NCIMB 13064 |
| 76 | Daniel | Pokorný | Structural and functional characterization of Ralstonia solanacearum lectin mutant |
| 21 | Tatyana | Prudnikova | Structural analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkanii USDA94 |
| 87 | David | Reha | Molecular dynamics comparison of E. coli WrbA apoprotein and holoprotein |
| 117 | Leona | Šerá | Pump-probe molecular dynamics (PPMD) simulations in GROMACS and its testing on the PDZ domain. |
| 51 | Katsiaryna | Shamayeva | Interdomain communication in the endonuclease/motor subunit of Type I restriction- modification enzyme EcoR124I |
| 46 | Tereza | Skálová | Structures of receptors and ligands of mammalian NK cells |
| 98 | Vojtěch | Spiwok | Enhancement of linear vs. non-linear motions in protein folding |
| 44 | Jan | Stránský | Critical parameters of S-SAD – a real case experience |
| 99 | Zoran | Šućur | Exploring free energy surface of oxytocin by using well-tempered metadynamics |
| 38 | Ekaterina | Tutubalina | Crystallization of FrpC protein |
| 61 | Petra | Zadravec | Development of lactic acid bacterium capable of binding Shiga toxin on the surface |
| 63 | Vojtěch | Zapletal | NMR study of haloalkane dehalogenase DhaA: resonance assignment and relaxation |
Deadline for registration and submission of abstracts was Friday February 21 2014
please, send your abstracts as attachment in word.docx format to kuzel-at-karlov.mff.cuni.cz
Deadline for the payment is Friday February 28 2014
Registered Participants of the XII Discussions
Abstracts of all contributions of the XII Discussions
Photos from the conference by Bohdan Schneider & Jan Stránský
… and by Radek Kuzel
The whole program takes place at Nový zámek.
The conference has been supported by program “Education for Competitiveness) of the Ministry of Education, Youth, and Sports of the Czech Republic.
Konference byla podpořena programem OP VK Ministerstva školství, mládeže a tělovýchovy.
INVESTMENTS IN EDUCATION DEVELOPMENT
Organizers of the meeting Bohdan Schneider, Radek Kužel, Ivana Kutá-Smatanová, Rudiger Ettrich, Petr Novák, Jindřich Hašek, Jan Dohnálek