XVIII Discussions in Structural Molecular Biology and 5th User Meeting of CIISB (Czech Infrastructure for Integrative Structural Biology)

You are invited to submit your abstract at https://www.xray.cz/setkani/abstracts.asp.
Submitted abstracts are available online.
Contributions in the form of short talk (20 min including discussion), long talk (30 min including discussion) or poster are welcome.

To contact the conference organizers, write to duskovaj@ibt.cas.cz.

Registration deadline: 28 February 2022
Abstract submission deadline: 28 February 2022

Congratulations to the winners of the Student competition!

Prize of the Czech Society for Structural Biology for an outstanding student presentation (in alphabetical order)

Ladislav Bartoš, Faculty of Science, Masaryk University
Designing transmembrane proteins to enhance transport of peptides across cell membranes
Markéta Šoltysová, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
How do SorC proteins recognize their DNA operators?
Zuzana Trebichalská, CEITEC, Masaryk University
In situ cryo-electron tomography of enterovirus cell entry

Prize of the Czech Society for Structural Biology for an outstanding student poster (in alphabetical order)

Stefana Njemoga, Institute of Neuroimmunology, Slovak Academy of Sciences
Multiapproach docking study for binding of intrinsically disordered tau peptides to monoclonal antibodies
Pavel Pohl, Institute of Physiology, Czech Academy of Sciences
14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains
Lucie Valentová, CEITEC, Masaryk University
A hunting strategy and virion structure of P. aeruginosa bacteriophage JBD30 revealed by cryo-electron microscopy

Arrival Instructions

Make sure you arrive with sufficient time left before the start of the program, as some of you have accommodation in a certain distance from the Conference venue.

For the main meals (breakfast, lunch, dinner) we must split into two groups. Your name tag will have a mark (a sticker or similar) which means that you dine downstairs in the restaurant of the Castle. No sticker – you dine on the main floor.

Currently, no Covid-related restrictions or measures are in place for the Conference.

We ask speakers to copy their presentation on the conference room computer well BEFORE the session and the chair persons to help speakers test their presentations. There will be strict timing of the lectures, unless marked otherwise in the program, you have 15 minutes to speak and 5 minutes for discussion.

The poster session in the Blue saloon (just in front of the entrance) will be held on Friday evening. The posters should be put up after Friday lunch and taken down after the poster session or on Saturday morning. Some poster boards will use pins, some glue tag (zvykacka), these will be provided.

Student competition

The Czech Society for Structural Biology invites all student presentations at the XVIII Discussions in Structural Molecular Biology and 5th User Meeting of CIISB to compete in two categories:
– Prize of the Czech Society for Structural Biology for an outstanding student poster
– Prize of the Czech Society for Structural Biology for an outstanding student presentation

The competing author must be a student (undergraduate or post-graduate studies) or a fresh PhD (less than one year from the defense of PhD thesis) at the time of the Discussions.

A maximum of three prizes will be awarded in each category at the end of the Conference. Each prize includes one book related to structural biology or molecular biology selected by the winner.

The event is organized by the Czech Society for Structural Biology, the Czech Infrastructure for Integrative Structural Biology, and the Institute of Biotechnology of the Czech Academy of Sciences.

We thank our sponsors for making this event possible:

Conference fees

Student 3300 CZK (2500 CZK without accommodation)
Non-student 4700 CZK (3900 CZK without accommodation)
Single occupancy accommodation – add 800 CZK
Members of CSSB will have the personal fee (300 CZK) subtracted from the paid amount.

The conference fee covers participation in the whole program, the printed book of abstracts for each participant, accommodation for two nights and full board (including breakfasts and coffee breaks).
The first meal is dinner on Thursday and the last meal is lunch on Saturday.

Programme

Thursday, March 24

11:30 – 13:30 Registration and accommodation, coffee

All talks are 20 minutes long unless indicated otherwise. All times are INCLUDING discussion.

14:00 Opening of XVIII Discussions and 5th User Meeting of CIISB (Jan Dohnálek, Vladimír Sklenář)

14:15 – 16:05 Session I Chair person: Jan Dohnálek

Zdeněk Lánský Institute of Biotechnology, Czech Academy of Sciences	Tau generates protective envelopes on microtubules L1 – 3
Miroslav Kloz Institute of Physics, Czech Academy of Sciences	Structural changes of carotenoid echinenone in Orange Carotenoid Protein studied by femtosecond Raman spectroscopy L2 – 4
Rohit Joshi Institute of Physiology, Czech Academy of Sciences	Nedd4-2 binding to 14-3-3 modulates the accessibility of its catalytic site and WW domains (Student talk) L3 – 5
Aneta Kozeleková CEITEC, Masaryk University	Phosphomimicking Mutations ≠ Phosphorylation – a case study of 14-3-3 protein (Student talk, CIISB user meeting) L4 – 5
Mark Johnson / Lukáš Gajdoš Institut Laue-Langevin, Grenoble	Neutrons for Structural Biology at the Institut Laue Langevin / Neutron diffraction for deciphering lectin-carbohydrate interactions in bacterial infection (30 min) L5 – 6

16:05 – 16:35 Coffee break

16:35 – 18:25 Session II Chair person: Pavlína Maloy Řezáčová

Ján Bíňovský CEITEC, Masaryk University	Baseplate structure of bacteriophage phi812 and mechanism of cell wall binding and penetration (Student talk, CIISB user meeting) L6 – 7
Zuzana Trebichalská CEITEC, Masaryk University	In situ cryo-electron tomography of enterovirus cell entry (Student talk, CIISB user meeting) L7 – 7
Miroslav Homola CEITEC, Masaryk University	Mechanism of virion formation of the Emiliania huxleyi virus 201 enveloped by two membranes (Student talk, CIISB user meeting) L8 – 8
Tomáš Kovaľ Institute of Biotechnology, Czech Academy of Sciences	Bacterial helicase-like transcription-associated factor HelD (CIISB user meeting) L9 – 8
Sebastian Zoll Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences	The structural basis of complement inhibition by the human parasite Trypanosoma brucei gambiense L10 – 9
Piotr Wardęga NanoTemper Technologies	Characterize your most challenging interactions. The New Monolith (10 min) L11 – 9

18:30 Dinner

20:00 CIISB Executive committee

Friday, March 25

7:30 – 9:00 Breakfast

9:00 – 10:50 Session III Chair person: Lukáš Žídek

Edward Curtis Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences	Supernova: a deoxyribozyme that catalyzes a chemiluminescent reaction L12 – 11
Maroš Huličiak Institute of Biotechnology, Czech Academy of Sciences	New Class of Structurally Robust Non-Antibody Protein Scaffolds for Directed Evolution (Student talk) L13 – 11
Jan Přibyl CEITEC, Masaryk University	Atomic force microscopy in structural biology (CIISB user meeting) L14 – 12
Petr Pompach Institute of Biotechnology, Czech Academy of Sciences	Structural Mass Spectrometry – An Advanced Tool in Protein Structure Analysis (CIISB user meeting) L15 – 13
Vilhelmiina Kontkanen Faculty of Science, University of South Bohemia	Structural Changes and Their Consequences for Azurin Oxidation in Vacuum and on Gold Interfaces L16– 13
Patrick King SPECION, s.r.o.	Microfluidic Modulation Spectroscopy (MMS): A Novel IR-Based Technique Providing Automated, Highly-Sensitive Protein Secondary Structure Characterisation in in situ Conditions (10 min) L17 – 14

10:50 – 11:10 Coffee break

11:10 – 12:50 Session IV Chair person: Pavel Plevka

Kristýna Adámková Institute of Biotechnology, Czech Academy of Sciences	Zinc-dependent nuclease from Stenotrophomonas maltophilia: Structural analysis of the active site (Student talk, CIISB user meeting) L18 – 15
Ondrej Cehlár Institute of Neuroimmunology, Slovak Academy of Sciences	Biophysical characterization of novel monoclonal antibodies targeting epitopes on the SARS-CoV-2 Spike protein (CIISB user meeting) L19 – 16
Martin Malý Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University Institute of Biotechnology, Czech Academy of Sciences	Integrative structural analysis of antibiotic-inactivating enzyme from Stenotrophomonas maltophilia (Student talk, CIISB user meeting) L20 – 17
Michal Koblížek Institute of Microbiology, Centre Algatech, Czech Academy of Sciences	2.4 Å structure of the double concentric ringed light harvesting complex from phototrophic bacterium Gemmatimonas phototrophica L21 – 18
Zuzana Hlavenková Thermo Fisher Scientific	Thermo Scientific™ Tundra Cryo-TEM: 100kV Cryo-TEM dedicated for Single Particle Analysis (10 min) L22 – 18

Poster flash talks, 2 minutes each:

• Alexandra Náplavová, Faculty of Science, Masaryk University, Multiple approaches for protein phosphorylation: a story of 14-3-3 (Student poster)
• Jan Šimek, Faculty of Science, Masaryk University, To homodimerize or to heterodimerize: story of 14-3-3 protein dimer formation (Student poster)
• Pavel Pohl, Institute of Physiology CAS, 14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains (Student poster)
• Olívia Petrvalská, Institute of Physiology CAS, 14-3-3 directly interacts with the kinase domain of CaMKK1 and inhibits calmodulin binding

12:50 – 14:00 Lunch

14:00 – 15:50 Session V Chair person: Vojtěch Spiwok

Martin Lepšík Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences	On the Importance of Physically Correct Models for Protein-Ligand Binding L23 – 19
Michal Malý Institute of Biotechnology, Czech Academy of Sciences	“ReDNATCO” – making DNATCO more useful for crystallographers L24 – 20
Ladislav Bartoš Faculty of Science, Masaryk University	Designing transmembrane proteins to enhance transport of peptides across cell membranes (Student talk) L25 – 21
Zdenek Futera Faculty of Science, University of South Bohemia	Mechanisms of Charge Transfer through Multiheme Protein Junctions, Their Distance and Band-Alignment Dependencies L26 – 22
Lubomír Rulíšek Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences	Exploring Reaction Mechanisms of Metalloproteins by Correlating Theory and Experiment L27 – 23
Martin Máša Beckman Coulter, Life Sciences Sales Dpt	EMnetik 24 System – DNA Cleanup for Genetic Engineering (10 min) L28 – 24

15:50 – 16:10 Coffee break

16:10 – 18:00 Session VI Chair person: Tomáš Kovaľ

Martin Marek Faculty of Science, Masaryk University	Can misfolded enzymes be beneficial? Yes, they can L29 – 25
Jitka Plucarová Faculty of Science, Masaryk University	Interactions of adaptor protein Grb2 with microtubule associated protein 2c (Student talk, CIISB user meeting) L30 – 25
Radim Novotný Faculty of food and biochemical technology, University of Chemistry and Technology	Structure and biological functions of TBEV Capsid protein (Student talk) L31 – 26
Raju Mandal Faculty of Science, Charles University	Biophysical characterization of the FOXO4:p53 complex (Student talk) L32 – 27
Dávid Tužinčin CEITEC, Masaryk University	Seeing the invisible – Study of transiently formed protein conformation found in domain 1.1 of bacterial transcription factor (Student talk, CIISB user meeting) L33 – 28

Poster flash talks, 2 minutes each

• Andreas Santamaria, Institut Laue-Langevin, Grenoble, Strikingly different roles of SARS-CoV-2 fusion peptides uncovered by neutron scattering (Student poster)
• Lucie Valentová, CEITEC, Masaryk University, A hunting strategy and virion structure of P. aeruginosa bacteriophage JBD30 revealed by cryo-electron microscopy (Student poster)
• Peter Pajtinka, CEITEC, Masaryk University, Synergistic antimicrobial activity of magainin 2 and PGLa revisited (Student poster)
• Patrícia Hrašnová, Institute of Neuroimmunology of Slovak Academy of Sciences, Structural and Biophysical Aspects of Lactoferrin and Its Interaction with Plasminogen (Student poster)
• Andrej Bitala, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Immunoprecipitation pull-down assay revealed binding between human CD160 and viral UL144 (Student poster)

18:00 – 19:00 General Assembly of the Czech Society for Structural Biology

The meeting will be held in the Czech and/or Slovak languages

1. Volba zapisovatele
2. Schválení programu
3. Výroční zpráva Rady ČSSB o činnosti a hospodaření
4. Zpráva revizní komise
5. Schválení výroční zprávy Rady ČSSB o činnosti a hospodaření
6. Infrastruktury se vztahem ke strukturní biologii, přístupy, financování, vývoj Instructu a jiných
7. Různé

19:00 – 20:00 Dinner

20:00 – 22:00 Poster session

Saturday, March 26

7:30 – 9:00 Breakfast

9:00 – 10:20 Session VII Chair person: Jiří Pavlíček

Václav Veverka Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences	A ubiquitous disordered protein interaction module orchestrates transcription elongation L34 – 28
Zuzana Cieniková CEITEC, Masaryk University	Structures of phage 812 neck suggest a mechanism for genome retention and release in Herelleviridae (CIISB user meeting) L35 – 29
Markéta Šoltysová Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences	How do SorC proteins recognize their DNA operators? (Student talk) L36 – 29
Vladena Bauerová Institute of Molecular Biology, Slovak Academy of Sciences	Structure-functional insights into the dantrolene binding site of the human cardiac ryanodine receptor: towards a deeper understanding of heart arrhythmias L37 – 30

10:20 – 10:40 Coffee break

10:40 – 12:20 Session VIII Chair person: Veronika Obšilová

Dalibor Košek Institute of Physiology, Czech Academy of Sciences	Mechanism of replicative “rolling-circle” DNA transposition in eukaryotes L38 – 31
Robert Vácha CEITEC, Masaryk University	How Viruses and Virus-like Nanoparticles Can Release Their Cargo/Genome (CIISB user meeting) L39 – 32
Tibor Füzik CEITEC, Masaryk University	Structure of tick-borne encephalitis virus immature particle solved by cryo-electron microscopy and sub-tomogram averaging (CIISB user meeting) L40 – 33
Richard Štefl CEITEC, Masaryk University	Cryo-EM of mouse RNase III–RNA complexes (CIISB user meeting) L41 – 34
Gabriel Demo CEITEC, Masaryk University	Cryo-EM ensemble reveals the mechanism of +1 ribosomal frame shifting L42 – 35

12:20 – 12:40 Evaluation of student presentations, prizes, concluding remarks

12:40 Lunch & departure