11-12 December 2017,
BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University, Průmyslová 595, Vestecwww.biocev.eu
Please accept our warm invitation to a conference/workshop “Proteins for Life” which is a title of the 12th meeting of P4EU – an international pan-European informal network of specialists in protein expression, purification and characterization, structural biologists, leaders of core facilities and research centres (www.p4eu.org). The program will consist of series of lectures highlighting the most recent advances within these fields, case studies and research developments, both from our international guests and from national community, with many possibilities for open informal discussions. Student talks are also welcome.
Program: Monday, 11th December – Conference Hall (U2.012)
| Time | Title of the talk | Speaker |
| 09:30 – 10:00 | Gathering and COFFEE | Outside conference hall |
| 10:00 – 10:20 | Program Overview & Welcome Address | Prof. Pavel Martásek, BIOCEV Director |
| 10:20 – 10:40 | P4EU Introduction | Dr. Sabine Supmann, Max-Planck Institute of Biochemistry |
| 10:40 – 11:10 | Combining cell-free protein production with nanoparticle technologies | Dr. Frank Bernhard, Goethe University Frankfurt |
| 11:10 – 11:30 | Adaptation of GoldenBac cloning system for FlashBac users | Dr. Peggy Stolt-Bergner, ProTech, Vienna Biocenter Core Facilities |
| 11:30 – 12:00 | Expression of IL-7 mutants modulating T-cell signaling in E. coli and P. pastoris | Dr. Ralf Paul, Medivir AB |
| 12:00 – 12:30 | The evolution of HEK293 expressions within the VIB Protein Core | Eng. Jurgen Haustraete, Protein Core, UGent – VIB |
| 12:30– 13:30 | LUNCH | BIOCEV Canteen |
| 13:30 – 14:00 | Rational design of CHO cell lines | Dr. Stefan Kol, Technical University of Denmark |
| 14:00 – 14:40 | KEYNOTE: Stronger, tighter, faster: improving protein stability, activity, and affinity through computational protein design | Dr. Sarel Fleishman, Weizmann Institute of Science |
| 14:40 – 15:10 | High-throughput expression of venom toxins in E. coli for drug discovery | Dr. Renaud Vincentelli, AFMB Marseille |
| 15:10 – 15:30 | Bovine antibody production and structural insight into the role of the light chain | Dr. Joanne Nettleship, Oxford Protein Production Facility |
| 15:30 – 16:00 | COFFEE BREAK | Outside conference hall |
| 16:00 – 16:30 | HTP protein production for drug discoveries, even small academic labs can hope to make money one day | Dr. Renaud Vincentelli, AFMB CNRS University Aix Marseille |
| 16:30 – 16:50 | Optimization of expression of extracellular 3’–nuclease/nucleotidase from Legionella pneumophila in E. coli | Dr. Tomáš Kovaľ, IBT CAS, BIOCEV |
| 16:50 – 17:10 | How to prepare your protein for crystallization trials | Dr. Pavlína Řezáčová, IOCB CAS |
| 17:10 – 17:30 | Adaptation of GoldenBac cloning system for FlashBac users | Dr. Peggy Stolt-Bergner, ProTech, Vienna Biocenter Core Facilities |
| 17:30 – 18:00 | Transient Gene Expression in Hi5 Insect cells | Joop van den Heuvel, Helmholtz Centre for Infection Research |
| 18:00 – 19:00 | Transfer to DINNER (P4EU/speakers) | Prague City Center |
Program: Tuesday, 12th December – Seminar Hall (U2.020A / U2.020B)
| Time | Title of the talk | Speaker |
| 09:30 – 10:00 | Gathering and COFFEE | Outside conference hall |
| 10:00 – 10:10 | BCDD PPT – Making Quality Proteins for Translational Research | Dr. Sharon Shepherd, University of Dundee |
| 10:10 – 10:30 | Thermofluor Assay: How to make your protein happy | Dr. Jana Škerlová, IOCB CAS |
| 10:30 – 11:00 | Guidelines to improve the quality of purified proteins: focused on antibodies | Dr. Hüseyin Besir, Head of R&D, Progen Biotechnik, Heidelberg |
| 11:00 – 11:30 | Structural mass spectrometry techniques for protein complexes | Dr. Petr Novák / Man, CMS BIOCEV |
| 11:30 – 12:00 | New structurally robust protein scaffolds for directed evolution | Dr. Bohdan Schneider, Institute of Biotechnology, CAS, BIOCEV |
| 12:00– 13:00 | LUNCH | BIOCEV Canteen |
| 13:00 – 15:00 | Tour around core facilities P4EU general discussion | P4EU members |
| 15:00 – 15:30 | COFFEE BREAK | Outside conference hall |
| 15:30 – 17:30 | Protein Characterization and Interaction Techniques Workshop | Centre of Molecular Structure, BIOCEV – room no. I1.022 |
| 17:30 – 18:00 | End of meeting, departure |