Proteins for Life – The 12th P4EU meeting

11-12 December 2017,
BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University, Průmyslová 595, Vestecwww.biocev.eu
Please accept our warm invitation to a conference/workshop “Proteins for Life” which is a title of the 12th meeting of P4EU – an international pan-European informal network of specialists in protein expression, purification and characterization, structural biologists, leaders of core facilities and research centres (www.p4eu.org). The program will consist of series of lectures highlighting the most recent advances within these fields, case studies and research developments, both from our international guests and from national community, with many possibilities for open informal discussions. Student talks are also welcome.

Program: Monday, 11th December – Conference Hall (U2.012)

TimeTitle of the talkSpeaker
09:30 – 10:00Gathering and COFFEEOutside conference hall
10:00 – 10:20Program Overview & Welcome AddressProf. Pavel Martásek, BIOCEV Director
10:20 – 10:40P4EU IntroductionDr. Sabine Supmann, Max-Planck Institute of Biochemistry
10:40 – 11:10Combining cell-free protein production with nanoparticle technologiesDr. Frank Bernhard, Goethe University Frankfurt
11:10 – 11:30Adaptation of GoldenBac cloning system for FlashBac usersDr. Peggy Stolt-Bergner, ProTech, Vienna Biocenter Core Facilities
11:30 – 12:00Expression of IL-7 mutants modulating T-cell signaling in E. coli and P. pastorisDr. Ralf Paul, Medivir AB
12:00 – 12:30The evolution of HEK293 expressions within the VIB Protein CoreEng. Jurgen Haustraete, Protein
Core, UGent – VIB
12:30– 13:30LUNCHBIOCEV Canteen
13:30 – 14:00Rational design of CHO cell linesDr. Stefan Kol, Technical University of Denmark
14:00 – 14:40KEYNOTE: Stronger, tighter, faster: improving protein stability, activity, and affinity through computational protein designDr. Sarel Fleishman, Weizmann Institute of Science
14:40 – 15:10High-throughput expression of venom toxins in E. coli for drug discoveryDr. Renaud Vincentelli, AFMB Marseille
15:10 – 15:30Bovine antibody production and structural insight into the role of the light chainDr. Joanne Nettleship, Oxford
Protein Production Facility
15:30 – 16:00COFFEE BREAKOutside conference hall
16:00 – 16:30HTP protein production for drug
discoveries, even small academic labs can hope to make money one day
Dr. Renaud Vincentelli, AFMB
CNRS University Aix Marseille
16:30 – 16:50Optimization of expression of
extracellular 3’–nuclease/nucleotidase
from Legionella pneumophila in E. coli
Dr. Tomáš Kovaľ, IBT CAS,
BIOCEV
16:50 – 17:10How to prepare your protein for crystallization trialsDr. Pavlína Řezáčová, IOCB CAS
17:10 – 17:30Adaptation of GoldenBac cloning system
for FlashBac users
Dr. Peggy Stolt-Bergner, ProTech,
Vienna Biocenter Core Facilities
17:30 – 18:00Transient Gene Expression in Hi5 Insect
cells
Joop van den Heuvel, Helmholtz
Centre for Infection Research
18:00 – 19:00Transfer to DINNER (P4EU/speakers)Prague City Center

Program: Tuesday, 12th December – Seminar Hall (U2.020A / U2.020B)

TimeTitle of the talkSpeaker
09:30 – 10:00Gathering and COFFEEOutside conference hall
10:00 – 10:10BCDD PPT – Making Quality Proteins for Translational ResearchDr. Sharon Shepherd, University of Dundee
10:10 – 10:30Thermofluor Assay: How to make your protein happyDr. Jana Škerlová, IOCB CAS
10:30 – 11:00Guidelines to improve the quality of
purified proteins: focused on antibodies
Dr. Hüseyin Besir, Head of R&D,
Progen Biotechnik, Heidelberg
11:00 – 11:30Structural mass spectrometry techniques for protein complexesDr. Petr Novák / Man, CMS BIOCEV
11:30 – 12:00New structurally robust protein scaffolds for directed evolutionDr. Bohdan Schneider, Institute of Biotechnology, CAS, BIOCEV
12:00– 13:00LUNCHBIOCEV Canteen
13:00 – 15:00Tour around core facilities
P4EU general discussion
P4EU members
15:00 – 15:30COFFEE BREAKOutside conference hall
15:30 – 17:30Protein Characterization and Interaction Techniques WorkshopCentre of Molecular Structure, BIOCEV – room no. I1.022
17:30 – 18:00End of meeting, departure
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