Biomolecular Interactions: Methods & Applications

Minisymposium on Biophysical Methods

24 th September 2018
Location: BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University (www.biocev.eu), Průmyslová 595, Vestec

Program: Conference Hall (U2.012)

08:30 – 09:00 Gathering and COFFEE Outside conference hall
09:00 – 09:15 Opening and Introduction Dr. Ondřej Vaněk, Charles University
09:15 – 09:50 Measuring Macromolecular Size-Distributions and Interactions at High Concentrations by Sedimentation Velocity Dr. Peter Schuck, NIH, Bethesda (Keynote lecture)
09:50 – 10:15 Studies of High-affinity Protein Interactions Using Analytical Ultracentrifugation with Fluorescence Detection Dr. Huaying Zhao, NIH, Bethesda
10:15 – 10:40 Introduction to Small Angle X‐ray Scattering (SAXS) and Its Use in Studying Protein-Protein Interactions Prof. Tomáš Obšil, Charles University

10:40 – 11:00 TEA & COFFEE BREAK Outside conference hall

11:00 – 11:25 Practical Strategies for the Characterization of Membrane Proteins by AUC Dr. Rodolfo Ghirlando, NIH, Bethesda
11:25 – 11:50 Small Angle Neutron Scattering for Membrane Proteins Dr. Christine Ebel, IBS, CNRS, Grenoble
11:50 – 12:15 Polymer-Encapsulated Nanodiscs for Membrane Biophysics Dr. Sandro Keller, TU Kaiserslautern

12:15 – 13:15 LUNCH BIOCEV Canteen

13:15 – 13:50 Validating Results of Molecular Interaction Measurements Across Different Experimental Platforms Dr. Grzegorz Piszczek, NIH, Bethesda (IUBMB lecture)
13:50 – 14:15 The Happy Partnership of Small-Angle Scattering and Hydrodynamics Prof. Olwyn Byron, University of Glasgow
14:15 – 14:40 ITC as a Tool in Biological Research: What Do We Need to Know? Basics, Planning, Data Treatment and Applications Dr. Margarida Bastos, University of Porto

14:40 – 15:00 TEA & COFFEE BREAK Outside conference hall

15:00 – 15:25 Microscale Thermophoresis and Additional Effects Measured with the NanoTemper Monolith Dr. Arthur Sedivy, VBCF, Vienna Biocenter
15:25 – 15:50 Studying Heat Shock Protein Dynamics and Interactions by Structural Mass Spectrometry Dr. Petr Man, IMB CAS, BIOCEV
15:50 – 16:15 Native mass spectrometry approaches for the investigation of structure and dynamics in complex biomolecular assemblies Dr. Dan Fabris, University at Albany

16:15 – 16:30 TEA & COFFEE BREAK Outside conference hall

16:30 – 19:00 Instrument demo, practical training and discussion (FEBS course only) BIOCEV CMS
19:00 – 22:00 Banquet dinner (FEBS course only) BIOCEV Canteen
20:00 – 22:00 Poster session (FEBS course only) BIOCEV Foyer

The minisymposium is organized as part of the FEBS Practical Course “Hydrodynamic and thermodynamic analysis of biological macromolecules and their interactions” and is co-sponsored by COST Action CA15126 ARBRE-MOBIEU (https://arbre-mobieu.eu) and Instruct.
The lectures are open free of charge – please, invite your students and colleagues as well!!!

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Nadrian C. Seeman: DNA, not Merely the Secret of Life

Professor Nadrian C. Seeman will visit IBT at BIOCEV on Monday, May 28. Do not miss his talk that he will deliver at 10 AM at the BIOCEV conference hall: DNA, not Merely the Secret of Life.

Prof. Seeman, Margaret And Herman Sokol Professor Of Chemistry at New Your University is a true pioneer of nanotechnology, his paper from year 1982 “Nucleic acid junctions and lattices” published in Journal of Theoretical Biology is outlining the concept of DNA nanotechnology. Prof. Seeman was inspired to explore self-assembling ability of DNA by Eschers’ woodcut. His open mind led to the first 3D nanoscale object, cube made of DNA (1991) and later on to the concepts now used in the technology of DNA origami. You can learn more about our distinguished guest in Wikipedia, https://en.wikipedia.org/wiki/Nadrian_Seeman

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Prague Protein Spring 2018

PPS 2018 aims to cover a thematic issue: “Proteins at Work” addressing concepts and system approaches demonstrated on selected general topics: “Proteins in living cells“,  “Large Scale organization of Proteins“, “Proteins in Biomineralization” and “Integrating knowledge about Proteins at the systems’ level”.

The conference will reflect both the theoretical and the experimental points of view, and finally, tackle a question of utilization of the knowledge in applied science. The major goal of PPS 2018 meeting is to propose a discussion on the current progress in the field and anticipate the future direction in protein science.

We are looking forward to welcome prominent scientists as well as young colleagues with significant contribution to the selected topics. The essential characteristics of this meeting is its dedication and focused mission delivered by highly acclaimed scientists and motivated students and their inter-generation interactions.

www.pragueproteinspring.cz

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