Biomolecular Interactions: Methods & Applications

Minisymposium on Biophysical Methods

24 th September 2018
Location: BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University (, Průmyslová 595, Vestec

Program: Conference Hall (U2.012)

08:30 – 09:00 Gathering and COFFEE Outside conference hall
09:00 – 09:15 Opening and Introduction Dr. Ondřej Vaněk, Charles University
09:15 – 09:50 Measuring Macromolecular Size-Distributions and Interactions at High Concentrations by Sedimentation Velocity Dr. Peter Schuck, NIH, Bethesda (Keynote lecture)
09:50 – 10:15 Studies of High-affinity Protein Interactions Using Analytical Ultracentrifugation with Fluorescence Detection Dr. Huaying Zhao, NIH, Bethesda
10:15 – 10:40 Introduction to Small Angle X‐ray Scattering (SAXS) and Its Use in Studying Protein-Protein Interactions Prof. Tomáš Obšil, Charles University

10:40 – 11:00 TEA & COFFEE BREAK Outside conference hall

11:00 – 11:25 Practical Strategies for the Characterization of Membrane Proteins by AUC Dr. Rodolfo Ghirlando, NIH, Bethesda
11:25 – 11:50 Small Angle Neutron Scattering for Membrane Proteins Dr. Christine Ebel, IBS, CNRS, Grenoble
11:50 – 12:15 Polymer-Encapsulated Nanodiscs for Membrane Biophysics Dr. Sandro Keller, TU Kaiserslautern

12:15 – 13:15 LUNCH BIOCEV Canteen

13:15 – 13:50 Validating Results of Molecular Interaction Measurements Across Different Experimental Platforms Dr. Grzegorz Piszczek, NIH, Bethesda (IUBMB lecture)
13:50 – 14:15 The Happy Partnership of Small-Angle Scattering and Hydrodynamics Prof. Olwyn Byron, University of Glasgow
14:15 – 14:40 ITC as a Tool in Biological Research: What Do We Need to Know? Basics, Planning, Data Treatment and Applications Dr. Margarida Bastos, University of Porto

14:40 – 15:00 TEA & COFFEE BREAK Outside conference hall

15:00 – 15:25 Microscale Thermophoresis and Additional Effects Measured with the NanoTemper Monolith Dr. Arthur Sedivy, VBCF, Vienna Biocenter
15:25 – 15:50 Studying Heat Shock Protein Dynamics and Interactions by Structural Mass Spectrometry Dr. Petr Man, IMB CAS, BIOCEV
15:50 – 16:15 Native mass spectrometry approaches for the investigation of structure and dynamics in complex biomolecular assemblies Dr. Dan Fabris, University at Albany

16:15 – 16:30 TEA & COFFEE BREAK Outside conference hall

16:30 – 19:00 Instrument demo, practical training and discussion (FEBS course only) BIOCEV CMS
19:00 – 22:00 Banquet dinner (FEBS course only) BIOCEV Canteen
20:00 – 22:00 Poster session (FEBS course only) BIOCEV Foyer

The minisymposium is organized as part of the FEBS Practical Course “Hydrodynamic and thermodynamic analysis of biological macromolecules and their interactions” and is co-sponsored by COST Action CA15126 ARBRE-MOBIEU ( and Instruct.
The lectures are open free of charge – please, invite your students and colleagues as well!!!

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