The practical course named “Advanced methods in macromolecular crystallization VIII“ will take place at the Academic and University Center at Nove Hrady (Czech Republic) from June 10th to June 16th, 2018. This crystallization course will be continuation of seven previously successfully organized FEBS combined practical and lecture and later practical FEBS courses. The planned course (for undergraduate, postgraduate students and postdocs with an interest in macromolecular crystallization) will be quite different from other established courses in protein crystallization, which are mostly of practical character and tend to neglect the basic theoretical aspects of crystal growth process. New trends and other methods will be discussed last day of the course and hereby the whole range of structural methods will be covered. The main aim and big advantage of the course is a healthy mixture of advanced discussions of the theory and of laboratory experiments. To achieve this goal, we have invited a number of prominent experts in the field as a teachers and tutors.
Proposed deadline for applications: March 31, 2018 For more information about the course, please have a look at the website https://macromolcryst2018.febsevents.org/ or contact the organizers. Ivana Kuta Smatanovae-mail: ivanaks@seznam.cz Pavlina Rezacovae-mail: rezacova@img.cas.cz Juan Manuel Garcia-Ruize-mail: jmgruiz@ugr.es
We would like to remind you about our invitation to an Instruct/CIISB course on fragment screening using crystallography laboratory equipment Dates: April 5-6, 2018 Venue: Centre of Molecular Structure, IBT CAS v.v.i., BIOCEV, Vestec, Prague-West, Czech Republic See the website: https://www.biocev.eu/event/instruct-ciisb-fragment-screening-course/ Apply before February 9 2018 to frederic.vellieux@ibt.cas.cz.
11-12 December 2017, BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University, Průmyslová 595, Vestecwww.biocev.eu Please accept our warm invitation to a conference/workshop “Proteins for Life” which is a title of the 12th meeting of P4EU – an international pan-European informal network of specialists in protein expression, purification and characterization, structural biologists, leaders of core facilities and research centres (www.p4eu.org). The program will consist of series of lectures highlighting the most recent advances within these fields, case studies and research developments, both from our international guests and from national community, with many possibilities for open informal discussions. Student talks are also welcome.
Program: Monday, 11th December – Conference Hall (U2.012)
Time
Title of the talk
Speaker
09:30 – 10:00
Gathering and COFFEE
Outside conference hall
10:00 – 10:20
Program Overview & Welcome Address
Prof. Pavel Martásek, BIOCEV Director
10:20 – 10:40
P4EU Introduction
Dr. Sabine Supmann, Max-Planck Institute of Biochemistry
10:40 – 11:10
Combining cell-free protein production with nanoparticle technologies
Dr. Frank Bernhard, Goethe University Frankfurt
11:10 – 11:30
Adaptation of GoldenBac cloning system for FlashBac users
Dr. Peggy Stolt-Bergner, ProTech, Vienna Biocenter Core Facilities
11:30 – 12:00
Expression of IL-7 mutants modulating T-cell signaling in E. coli and P. pastoris
Dr. Ralf Paul, Medivir AB
12:00 – 12:30
The evolution of HEK293 expressions within the VIB Protein Core
Eng. Jurgen Haustraete, Protein Core, UGent – VIB
12:30– 13:30
LUNCH
BIOCEV Canteen
13:30 – 14:00
Rational design of CHO cell lines
Dr. Stefan Kol, Technical University of Denmark
14:00 – 14:40
KEYNOTE: Stronger, tighter, faster: improving protein stability, activity, and affinity through computational protein design
Dr. Sarel Fleishman, Weizmann Institute of Science
14:40 – 15:10
High-throughput expression of venom toxins in E. coli for drug discovery
Dr. Renaud Vincentelli, AFMB Marseille
15:10 – 15:30
Bovine antibody production and structural insight into the role of the light chain
Dr. Joanne Nettleship, Oxford Protein Production Facility
15:30 – 16:00
COFFEE BREAK
Outside conference hall
16:00 – 16:30
HTP protein production for drug discoveries, even small academic labs can hope to make money one day
Dr. Renaud Vincentelli, AFMB CNRS University Aix Marseille
16:30 – 16:50
Optimization of expression of extracellular 3’–nuclease/nucleotidase from Legionella pneumophila in E. coli
Dr. Tomáš Kovaľ, IBT CAS, BIOCEV
16:50 – 17:10
How to prepare your protein for crystallization trials
Dr. Pavlína Řezáčová, IOCB CAS
17:10 – 17:30
Adaptation of GoldenBac cloning system for FlashBac users
Dr. Peggy Stolt-Bergner, ProTech, Vienna Biocenter Core Facilities
17:30 – 18:00
Transient Gene Expression in Hi5 Insect cells
Joop van den Heuvel, Helmholtz Centre for Infection Research
18:00 – 19:00
Transfer to DINNER (P4EU/speakers)
Prague City Center
Program: Tuesday, 12th December – Seminar Hall (U2.020A / U2.020B)
Time
Title of the talk
Speaker
09:30 – 10:00
Gathering and COFFEE
Outside conference hall
10:00 – 10:10
BCDD PPT – Making Quality Proteins for Translational Research
Dr. Sharon Shepherd, University of Dundee
10:10 – 10:30
Thermofluor Assay: How to make your protein happy
Dr. Jana Škerlová, IOCB CAS
10:30 – 11:00
Guidelines to improve the quality of purified proteins: focused on antibodies
Dr. Hüseyin Besir, Head of R&D, Progen Biotechnik, Heidelberg
11:00 – 11:30
Structural mass spectrometry techniques for protein complexes
Dr. Petr Novák / Man, CMS BIOCEV
11:30 – 12:00
New structurally robust protein scaffolds for directed evolution
Dr. Bohdan Schneider, Institute of Biotechnology, CAS, BIOCEV
12:00– 13:00
LUNCH
BIOCEV Canteen
13:00 – 15:00
Tour around core facilities P4EU general discussion
P4EU members
15:00 – 15:30
COFFEE BREAK
Outside conference hall
15:30 – 17:30
Protein Characterization and Interaction Techniques Workshop
Centre of Molecular Structure, BIOCEV – room no. I1.022
We are happy to inform you that “XV Discussions in Structural Molecular Biology and the 2nd User Meeting of the Czech Infrastructure for Integrative Structural Biology”, the next annual meeting of the Society will be held in Nové Hrady, March 22 – 24, 2018. More information about the conference at the conference page.
